Investigation of skin microbiota reveals Mycobacterium ulcerans-Aspergillus sp. trans-kingdom communication

Nassim Hammoudi *1, Cassagne Carole 2, Matthieu Million 1, Stephane Ranque 2, Michel Drancourt 2, Odilon Kabore 1, Dezemon Zingue 1;3, Amar Bouam 2

Author address: 

1 Aix Marseille Univ., IRD, MEPHI, IHU Méditerranée Infection, Marseille, France, IHU Méditerranée Infection, Marseille, France; 2 Aix Marseille Univ., IRD, AP-HM, SSA, VITROME, IHU Méditerranée Infection, Marseille, France; 3 Centre Muraz, Bobo-Dioulasso, Burkina Faso

Abstract: 

Background: Mycobacterium ulcerans secretes a series of non-ribosomal-encoded toxins known as mycolactones that are responsible for causing a disabling ulceration of the skin and subcutaneous tissues named Buruli ulcer. The disease is the sole non-contagious mycobacteriosis among the three most common mycobacterial diseases in humans. Direct contact with contaminated wetlands is a risk factor for Buruli ulcer, responsible for M. ulcerans skin carriage before transcutaneous inoculation with this opportunistic pathogen.

Materials/methods: Initially, we used molecular biology to study the possibility of asymptomatic skin carrying of M. ulcerans in farmers in Burkina Faso using two IS2404 IS2606 insertion sequences and the Kr-b genes specific to M. ulcerans. In a second step, we analysed the bacterial and fungal skin microbiota in individuals exposed to M. ulcerans in Burkina Faso. Then, we cultured the skin microbiota of asymptomatic M. ulcerans carriers and negative control individuals, all living in the region of Sindou.

Results: We showed that M. ulcerans-specific DNA sequences were detected on the unbreached skin of 6/52 (11.5%) asymptomatic farmers living in Sindou versus 0/52 (0%) of those living in the non-endemic region of Tenkodogo. A total of 84 different bacterial and fungal species were isolated, 21 from M. ulcerans-negative skin samples, 31 from M. ulcerans-positive samples and 32 from both. More specifically, Actinobacteria, Aspergillus niger and Aspergillus flavus were significantly associated with M. ulcerans skin carriage. We further observed that in vitro, mycolactones induced spore germination of A. flavus, attracting the fungal network.

Conclusions: These unprecedented observations suggest that interactions with fungi may modulate the outcome of M. ulcerans skin carriage, opening new venues to the understanding of Buruli ulcer pathology, prophylaxis and treatment of this still neglected tropical infection.

Presenter email address: [email protected]

2020

abstract No: 

6323

Full conference title: 

European Congress of Clinical Microbiology and Infectious Diseases 2020
    • ECCMID 30th (2020)