Investigating the yet8208;unknown biomass degrading and modifying enzymes of Aspergillus oryzae  

Ref ID: 18511

Author:

George Anasontzis,
Gianni Panagiotou,
Lisbeth Olsson

Author address:

Department of Chemical and Biological Engineering, Industrial Biotechnology, Chalmers University of Technology,
SE-412 96 Gothenburg, Sweden  
Center for Biological Sequence Analysis, Department of Systems Biology,
Technical University of De

Full conference title:

11 th European Conference on Fungal Genetics

Abstract:

The associations of plant cell wall components with A. oryzae’s extracellular enzyme machinery was investigated
from a chemical perspective using an integrated analysis of the transcriptome profile.
Strain RIB40 of A. oryzae was cultured on various carbon sources, namely cellohexaose, mannohexaose,
xylopentaose, arabinoheptaose, glucohexaose, glucosyl maltotriosyl maltotriose, galactosyl mannotriose, turanose
and sophorose, and the transcribed genes were determined with DNA microarrays. The statistically significant
genes were selected and novel hydrolases were identified, which would be further heterologously expressed for
subsequent characterization. This work represents a novel way of integrating computational chemical biology and
classical enzyme research for improving lignocellulose bioconversion.
More specifically, we aim at the heterologous expression and characterization of seven different hypothetical and
non classified proteins of A. oryzae, which could prove to be useful tools in the wood biomass separation and
modification process.

Abstract Number: PR8.4

Conference Year: 2012

Link to conference website: http://www.ecfg.info/images/Abstract_Book_Electronic.pdf

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