Intrafungal concentration of posaconazole

Ref ID: 17769

Author:

F. Farowski*, A. Hamprecht, C. Müller, A. Steinbach, J.J. Vehreschild,
M.J.G.T. Vehreschild, O.A. Cornely

Author address:

(Cologne, DE)

Full conference title:

22nd European Congress of Clinical Microbiology and Infectious Diseases

Abstract:

We recently showed that various antifungal drugs, i.e. anidulafungin,
micafungin, and posaconazole, accumulate within cells of the peripheral
blood. The ratio between the cellular and the extracellular (C/E)
concentration depends on the composition, i.e. the protein concentration,
of the surrounding medium. However, the composition of the
surrounding medium might also influence the antifungal concentration
within the fungi themselves and significantly contribute to the efficacy
of these drugs. Hence, we currently develop a method to determine
intrafungal antifungal concentrations. We present data on the ’’intrafungal
concentration’’ of posaconazole within Candida albicans.
Method: Suspensions of Candida albicans were prepared in RPMI-
1640 medium and incubated with different concentrations of
posaconazole (33-990 ng/mL). Following an incubation period of
1 hour the drug was removed by two consecutive washings with sterile
water. Afterwards, cell counts were determined using a Neubauer
chamber and samples were stored at )80C until use. The intrafungal
concentrations were determined by a chromatography tandem mass
spectrometry (LC-MS/MS) method similar to the previously described
method for the quantitation of different antifungals within human
peripheral blood cells. An additional step to concentrate the samples
was added to increase the sensitivity of the method.

Abstract Number: NULL

Conference Year: 2012

Link to conference website: NULL

New link: NULL

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