Inter-Laboratory and Inter-Study Reproducibility of a Lateral Flow Device (LFD) for the Detection of Invasive Pulmonary Aspergillosis (IPA)

Ref ID: 18693


N. P. Wiederhold, PharmD – Associate Professor1,2, L. K. Najvar, BS – Laboratory Director 2,3, R. A. Bocanegra, BA – Research Associate 2,3, W. R. Kirkpatrick, MS – Project Coordinator 2,3, T. F. Patterson, MD – Professor 2,3, C. R. Thornton, PhD – S

Author address:

1UT Austin, Austin, TX, 2UTHSCSA, San Antonio, TX, 3STVHCS, San Antonio, TX, 4Univ. Exeter, Exeter, United Kingdom.

Full conference title:

52nd Annual ICAAC

Date: 9 September 2014


Background: The potential utility of a LFD for the early detection of IPA has been demonstrated (Wiederhold et al. Clin Vaccine Immunol 2009). Our objective was to evaluate the inter-laboratory (UT Austin and Univ. Exeter) and inter-study reproducibility of this LFD. Methods: An established neutropenic guinea pig model of IPA was used for pulmonary inoculation with Aspergillus fumigatus. At predetermined time points (1 hr, 3, 5, and 7 days post-inoculation) blood and BAL fluid were collected from infected and uninfected animals. An Aspergillus glycoprotein antigen was detected by each laboratory independently using an IgG monoclonal antibody-based LFD by applying 0.1mL of sample to the device. Galactomannan (GM) and (1,3)-β -D-glucan (BG) were measured using commercially available kits. The results were compared to those from a previous study. Results: Good inter-laboratory agreement was observed with the LFD. Overall, 96.7 % (29/30) of the serum and 73.3% (22/30) of the BAL samples from infected guinea pigs were in agreement between the two laboratories. The results for both serum and BAL samples from uninfected animals were 100% concordant. Good inter-study agreement was also found, as the LFD results observed in the current study were comparable to previous results (Table). In addition, the LFD results in both serum and BAL were similar to those observed with the BG and GM assays. Conclusions: These results demonstrate that the LFD assay is reproducible between different laboratories and studies. This assay leads to consistent and specific results with serum and BAL fluid for the detection of IPA.

Abstract Number: M-1682

Conference Year: 2012

Link to conference website: NULL

New link: NULL

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