INSULATION ASPERGILLUM Spp . WITH VEGETARIAN FOOD

Ref ID: 19483

Author:

I Zurak1*

Author address:

1Microbiology, Hospital/University, Zagreb, Croatia

Full conference title:

6th Advances Against Aspergillosis 2014

Abstract:

Purpose:
Various species of the genus Aspergillums are responsible for causing opportunistic infection in
both humans and a wide range of animals. Early diagnosis is critical for effective treatment and for
avoiding inappropriate administration of costly toxic antifungal therapy. The diagnosis bee mainly
based on traditional culture methods. Fruits and vegetables are rich in vegetable care in protein,
vitamins, and rich in carbohydrate, ferment and electrolytes and are not surprising that there is
deterioration and increase the appearance of various types of mold, fungi and so on. The aim of this
study was to examine various types of fruits and vegetables in preparation for feeding insulation
Aspergillums species.
Methods:
We used the first singles various types of fruits and vegetables, which are after fragment with mixer
and underwent maceration in solution electrolyte, pasteurizing performed at 56/60°C for 12-18h.
As electrolyte, mixture been used: Sodium chloride, Cupric sulfate, Magnesium sulfate, Ferric
ammonium citrate and Potassium metabisulfite. After pasteurization content, we spat as a tea (no
filter), add 20g/l agar heated to boiling and poured into Petri dishes. We decided on the type of fruit
and vegetables that have shown a good port Aspergillums species, especially if they are shown and
selective activity and bee not allowed to increase Gram positive and negative types. We decided on
a few well-chosen types of fruits and vegetables, which are supplemented agar made feeding the
isolation of Aspergillums species from clinical samples. In parallel, we used the classical method of
isolation using Sabouraud agar. Samples been incubated at 24-370C for 18-24h.
Results:
The results were very successful and have shown that the tendency on vegetable and fruit can
bee used as a medium in the isolation of Aspergillums species. Medium was without necessity
add antibiotics to achieve selectivity. Fruit gives a much better isolation of Aspergillums species
of vegetables. Increase and thus insulating Aspergillums species is much faster and better than on
Sabouraud agar.
Conclusion:
Vegetables and fruits in particular offer great opportunities isolation of fungi, especially those that
are sensitive to antibiotics and selective additions that today use in Sabouraud agar. Arrive faster to
the results of what is important in the treatment of infections caused by fungal diseases. Manufacturer
dehydrated feeding in microbiology should pay attention to the production of feeding from fruits
and vegetables.

Abstract Number: 11

Conference Year: 2014

Link to conference website: http://www.AAA2014.org

New link: NULL

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