Ref ID: 18469
Author:
Lic Manuel Sanguinetti, Sotiris Amillis, Dr Claudio Scazzocchio, Sergio Pantano, Ana Ramón
Author address:
Sección Bioquímica, Departamento de Biología Celular y Molecular, Facultad de Ciencias, UdelaR, Montevideo,
Uruguay Departamento de Botánica, Facultad de Biología, Universidad de Atenas, Grecia Grupo de
Biosimulaciones, Institut Pasteur de Montevid
Full conference title:
11 th European Conference on Fungal Genetics
Abstract:
UreA, is a high8208;affinity urea/H+ symporter which seems to be the sole transport system specific for urea
inAspergillus nidulans. Homologous proteins have been characterized in Saccharomyces cerevisiae, Paxillus
involutus, Candida albicans, in the model plant Arabidopsis thaliana and more recently in Oryza
sativa (rice).Paralogues are present in the genomic sequences of all Aspergilli. The protein is predicted to consist of
15 transmembrane helical domains (TMSs), with an extracellular N8208;terminus and an intracellular C8208;terminus.
Little is known about the structure8208;function relationship of these membrane proteins. In order to address this
subject, we designed a mutational strategy based on 3D homology modelling of UreA and the identification of
conserved residues in all known fungal urea transporters. The functionality of the mutant proteins was assayed by
growth tests on urea and resistance to its toxic analogue, thiourea. All mutations were introduced on an UreA::GFP
fusion construct, which allowed us to follow the sub8208;cellular localization of mutant fusion proteins. This strategy
allowed us to identify a number of key residues involved in the recognition and/or translocation of urea across the
membrane. These mutations localize in helixes number 3, 7 or 11 which, according to homology modelling, are
predicted to be part of the substrate binding domain. A chemical mutagenesis approach has been also undertaken
which allowed for the identification of key residues for the functionality of the protein.
Our work constitutes the first mutational analysis in this family of transporters, providing insights into urea
transporters functionality.
Abstract Number: PR1.61
Conference Year: 2012
Link to conference website: http://www.ecfg.info/images/Abstract_Book_Electronic.pdf
New link: NULL
Conference abstracts, posters & presentations
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Title
Author
Year
Number
Poster
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v
C Gutierrez Perez1*, S Dhingra1,2, SM Kwansy3, TJ Opperman3, RA Cramer1
2022
93
-
v
PC Chatterjee1*, WC Chiu1, EBB Burgener2, PLB Bollyky3, JH Hsu1
2022
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v
AS Serris1*, FU Uhel2, GMB Martin-Blondel3, OL Lambotte4, MEB Bougnoux5, EL Litvinova6, AO Ouedrani7, CR Rouzaud1, VB Bedarida8, JHR Raphalen9, FL Lanternier1,10
2022
91
-
v
ZA Lewis1, RB Meagher2*, S Ambati2, X Lin1
2022
90
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v
QJ Choudhury1*, S Ambati2, X Lin1, ZA Lewis1, RB Meagher2
2022
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v
M James1*, KW Liu1, E Vesley1, T Hohl2, RA Cramer1
2022
88
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v
D Luptakova1, RH Patil1,2, V Havlicek1,2*
2022
87
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v
EI Matthaiou1*, W Chiu1, C Conrad2, J Hsu1
2022
86
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v
H Kamboj*, M Vijay, L Gupta, P Vijayaraghavan
2022
85
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S Hartung1,2*, TNM Hoang1,2*, Z Cseresnyes3*, N Schuck1, K Rennert4,5, F Klos6, AS Mosig5,7, MT Figge3,8, M von Lilienfeld-Toal1,2
* Authors 1, 2 and 3 contributed equally
2022
84
