Inflammatory responses in mice after intranasal instillation of spores of some aspergillus and penicillium spp monitored in Southwest Nigeria


Adeyinka Odebode, Adedotun Adekunle, Ebenezer Farombi

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European Academy of Allergy and Clinical Immunology Congress 2020


Fungi are an increasing public health problem worldwide because they have a great impact on human health. In this study, comparative analysis of airborne fungal spores for two years in ten different locations in Lagos and Oyo States, Nigeria. Identification and characterization of fungal species was carried out by amplification of internal transcribed spacer 1 and 4 gene followed by quantification of allergenic gene by reverse transcriptase quantitative polymerase chain reaction in the most abundant fungal isolates.

A mouse model was devised to elucidate and compare the adverse effects provoked by the four most abundant fungi isolated from various locations in all locations. Fifty-four Balb/b albino mice were grouped into nine treatments of six per cage with a control group. The animals were exposed intranasally to the spores of A.flavus, A. penicilloides, Penicillium citrinum and Penicillium chrysogenum at 2.3 x 10 and 3.2 x 10 m/L. Both dose-response and time-course inflammatory and toxic responses were investigated after a single dose of the microbes.

Biochemical parameters and histopathology revealed that all the microbes studied provoked inflammation after a single dose but the magnitude and its characteristic features were different. The spores of A.flavus, and A. penicilloides provoked a very intense acute inflammation indicated by production of increased malondialdehyde, myeloperoxidase, total protein production in the lungs, recruitment of inflammatory cells into the airways and expression of neutrophils, eosinophils and basophils in the blood. The inflammatory cell response in the lungs was more severe and varies with each organism at different concentrations. Histopathology result for all inoculated organisms on mice lung showed that there was mild thickening of the alveolar interstitium and moderate haemorrhages. There was also accumulation of inflammatory cells around blood vessels suggestive of vasculitis. The results showed that A. penicilloides in addition to A. flavus had highly significant lethality on lungs of mice than those of P. citrinum and P. chrysogenum

Data on the abundance/prevalence of fungi species in the atmosphere of sub-Saharan Africa is limited which necessitated this study for forecasting the prevalence of allergenic fungi in the environment at various seasons. It was inferred in the study that Aspergillus and Penicillium species can be major contributors to allergy/ asthma in the study areas

Abstract Number: 0079

Conference Year: 2020

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EAACI 2020

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