Induction of Oxidative stress response in Aspergillus fUMigatus when exposed to Caspofungin

Ref ID: 19514

Author:

A Eshwika1*, A Smith1, J Fallon1, J Kelly1, K Kavanagh1

Author address:

1Biology, NUI-M, Maynooth, Ireland

Full conference title:

6th Advances Against Aspergillosis 2014

Abstract:

Purpose:
The purpose of this work was to establish if Caspofungin induced an oxidative stress in Aspergillus
fumigatus
Methods:
Proteomic analysis was performed to identify proteins altered in expression following exposure of
A. fumigatus to caspofungin. The activity of a variety of enzymes was analysed.
Result:
Exposure of A. fumigatus to 0.1 or 1.0 μg/ml caspofungin for 4 hours resulted in protein leakage from
hyphae and the increased expression of a variety of proteins involved in the oxidative stress response
(e.g. catalase, antibiotic response protein), virulence (e.g. Asp F3, 18kDa antigen) and homeostasis
(e.g. glyceraldehyde 3-phosphate, translation elongation factor, ATP synthase F1). In addition there
was an increase in the activity of catalase, glutathione reductase and superoxide dismutase in hyphae
exposed to 0.1 μg/ml caspofungin for 1 hour.
Conclusion:
The results indicate that when non-growing cultures of A. fumigatus were exposed to caspofungin
there was an increase in the expression of a number of proteins associated with virulence and the
oxidative stress response, and the activity of detoxifying enzymes possibly in an attempt by the
fungus to circumvent the intracellular effects of the antifungal agent.

Abstract Number: 42

Conference Year: 2014

Link to conference website: http://www.AAA2014.org

New link: NULL

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