Incidence of breakthrough fungal infection during primary antifungal prophylaxis in acute myeloid leukoemia patients in a cancer centre

Ref ID: 17773

Author:

M.Z.R. Gomes*, R.E. Lewis, P.M.C.M. Farias, C. Wu,
D.P. Kontoyiannis

Author address:

Houston, US)

Full conference title:

22nd European Congress of Clinical Microbiology and Infectious Diseases

Abstract:

Objectives: To investigate the patterns of primary antifungal
prophylaxis (PAP) use and the incidence of breakthrough fungal
infection (BFI) in patients with acute myeloid leukemia (AML) in a
cancer center.
Methods: We analyzed 75 unselected patients with newly diagnosed
AML admitted to MD Anderson Cancer Center who received PAP
through August 2009 to March 2011.localizes in cell nucleus (Fig.1). NS1 protein could induce apoptosis in
human lung epithelial cell line (NCI-H292) (Figs 2 and 3). Cytochrome
C release could be observed dramatically in NS1 transfected cells and
the effect was enhanced by apoptosis inducer (Fig. 4). Taken together,
these data indicate that influenza A virus NS1 protein serves as a strong
inducer of apoptosis in human lung epithelial cells and triggers
apoptosis could be via mitochondria-dependant intrinsic pathwayFig. 1. Analysis the expression and localization of NS1 protein in
H1299 cell line by immunofluorescence and confocal assay.
The pXJ40-HA-NS1 transfected H1299 cell staining with anti-HA
serum and Alexa488 conjugated secondary antibody (A), anti-Cytochrome
C MAb and Alexa555 conjugated secondary antibody (B),
DAPI dye (C), respectively; Merged image (D).
Fig. 2. Cytotoxicity detection by MTT assay on NCI-H292 cells
transfected by pXJ40-HA-NS1 for 24h. A:vector;B: STS 0.025 nmol;C:
NS1;D: NS1 + STS 0.025 nmol.
Fig. 3. Identification of apoptosis by FCM assay of Annixin-V/PI
double staining.
A:vector;B: NS1;C: STS0.025 nmol;D: NS1 + STS0.025 nmol.
Fig. 4. Cytochrome C release detection by WB assay in NCI-H292 cells
transfected by pXJ40-HA-NS1 and induced by STS (0.025 nmol) for
24 hour.

Abstract Number: NULL

Conference Year: 2012

Link to conference website: NULL

New link: NULL

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