In vivo imaging of rat experimental invasive pulmonary aspergillosis using fibred confocal fluorescence microscopy

Ref ID: 18416

Author:

Helene Morisse, Loraine Heyman, Mathieu Salaun, Loic Favenec, Jean
Michel Picquenot , Pierre Bohn1, Luc Thiberville

Author address:

Laboratoire QuantF LITIS,
EA 4108, Faculté de Medecine et de Pharmacie, Rouen, France; Laboratoire de
Parasitologie Expérimentale, EA 3234, Faculté de Medecine et de Pharmacie,
Rouen, France; Service d’Anatomopahtologie, Centre Henri Becquerel, Ro

Full conference title:

European Respiratory Congress

Abstract:

Rational: Aspergillus fumigatus is responsible for life-threatening respiratory infections in immunosuppressed patients. Early diagnostic would improve the
prognosis. Fibred confocal fluorescence microscopy (FCFM) is a new endoscopic
tool that enables in vivo microscopic imaging of the distal lung in situ. In this
study we tested the hypothesis that FCFM could be utilized for the visualization
of pulmonary aspergillosis infection in vivo, in situ.
Methods: Experimental pulmonary invasive fungal infections were induced in
immunosuppressed rats using a wild strain of A.fumigatus (n=6) a wild strain of
Geosmithia argilacea (n=6) and a fluorescent transformed Tag-RFP A.Fumigatus
strain (n=6). Subpleural areas of pulmonary infection and control lungs were
imaged in vivo using FCFM through a chest wall window.
Results: From the fungal strains, only hyphae of the Tag-RFP A.fumigatus were
detectable by FCFM both in vitro and in vivo. In vivo, hyphae of the Tag-RFP
Aspergillus strain were visualized by FCFM in 100% of the lung infection with
a specificity of 100% (6/6) compared to normal lung and other fungal infection.
Using non fluorescent strains, a specific local infiltration of fluorescent cells could
be localized in half fungal subpleural microabcesses (Se 100%;Spe 58%).
Conclusion: FCFM represents the first imaging technique of pulmonary aspergillosis
in real time and in vivo. It provides a new tool to study host-pathogen
interactions and may help for early diagnosis of pulmonary aspergillosis in vivo.

Abstract Number: P2522

Conference Year: 2011

Link to conference website: http://www.ers-education.org/ersMade/abstract_print_11/files/Abstract_book_2011.pdf

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