In vitro checkerboard data correlate with in vivo outcome of polyene+azole combination therapy against experimental candidiasis

Ref ID: 17729

Author:

A. Elefanti*, M. Siopi, P. Verweij, J.W. Mouton, J. Meletiadis

Author address:

(Athens,
GR; Nijmegen, NL)

Full conference title:

22nd European Congress of Clinical Microbiology and Infectious Diseases

Abstract:

Objectives: In vivo/in vitro correlation of antifungal combination
testing is necessary in order to reliably assess the efficacy of
combination regimens. We therefore attempted to correlate in vitro
checkerboard testing of posaconazole (POS) and amphotericin B
(AMB) with the in vivo outcome of combination therapy against
experimental candidiasis in a neutropenic murine model.
Methods: For, the in vitro experiments, serial twofold drug dilutions
were prepared in 96-well microtitration plates at concentrations of
0.008-0.5 mg/L for AMB and 0.00006-0.06 mg/L for POS alone and
in combination (8 · 12 checkerboard) in quadruplicate. After
inoculation with 2.5 · 103 CFU/mL of a Candida albicans clinical
isolate, plates were incubated for 48 hours at 37C and read
spectrophotometrically at 405 nm. For the in vivo experiments 4-
6 weeks old CD1 female mice were rendered neutropenic before i.v.
infection with 105 CFU/mouse of the C. albicans isolate. In vivo 3 · 3

Abstract Number: NULL

Conference Year: 2012

Link to conference website: NULL

New link: NULL

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