Immuno-clinical investigation of IgEmediated cross reactivity of major nosocomial fungal allergens: a serious health hazard in Barasat District Hospital

Author:

Saha M 1; Bhattacharya K 2

Author address:

1 Basirhat College, Basirhat, India; 2 Department of Botany, Visva Bharati (a central University), Santiniketan, India

Full conference title:

European Academy of Allergy and Clinical Immunology Congress 2017

Abstract:

Introduction: Fungal spores are one of the major types of microorganisms, can be present in all hospital environments, may be transmitted through air, patients and air conditions. It appears that adequate information on the nosocomial fungal allergy in hospital environment of West Bengal are largely lacking though 10% of the patients’ infections are suspected to be hospital-acquired.

Objectives: This study aimed to identify the novel IgE reactive proteins of nosocomial fungal allergens found in different wards of Barasat District Hospital, WB and investigate the in-vitro cross-reactivity among these allergens by in-vitro immunoenzymatic biochemical methods.

Results: Andersen two stage sampler and Burkard sampler trapped a total of 22 fungal genera belonging to 34 species from different wards of the hospital. The most predominant air borne fungi have been identified are Aspergillus group, showed the highest (21.9%) prevalence of sensitization when tested (by SPT) on 128 nosocomial infected atopic patients, followed by Alternaria alternata(AA), Cladosporium cladosporioides(CC), Curvularia lunata(CL), Candida albicans (CA), Penicillium citrinum(PC), Epicoccum nigrum(EN), Tricophyton rubrum(TR), Fusarium oxysporum(FO) showed 6, 9, 9, 8, 7, 10, 8 and 6 IgE specific reactive proteins in western immunoblotting using a panel of sera from immunotherapy free subjects, though these antigens were profiled into numerous distinct bands in 11% SDS PAGE. ELISA inhibition has been used to identify degree of cross-reactivity among fungi whereas immunoblot-inhibition elucidate a componentbased shared allergens. IgE-immunoblot inhibition showed proteins of 26, 38, 45 and 50 kDa of CL were inhibited by AA and EN whereas A. fumigatus(AF) inhibited 26, 45 and 50 kDa proteins. Further 58 kDa glycoprotein of AF showed cross reactivity with 55 kDa antigen of CA and PC showed shared components of 34 kDa from A. fumigatus(AF) and EN. PC, CC and FO also shared multiple antigenic determinants.

Conclusions: This study is alarming for asthmatic nosocomial hazards in this Govt. hospital. Identification of IgE specific reactive components of predominant fungal allergens and cross-reactivity among each other, delined in this study could minimize the hazard of therapeutic and diagnostic use of these cross-reactive components, in fungal allergen-specific immunotherapy.

Abstract Number: 0419

Conference Year: 2017

Link Conference abstract: 

EAACI 2017

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