Identification of protein kinase A target genes of Aspergillus fumigatus by functional genomics

Ref ID: 18258

Author:

J. Macheleidt
, W. Schmidt-Heck
, T. Heinekamp
, A. Brakhage

Author address:

Hans-Knoell-Institute, Molecular and Applied Microbiology, Jena,
Germany
Hans-Knoell-Institute, Systems Biology / Bioinformatics, Jena, Germany

Full conference title:

Asperfest 9

Abstract:

A. fumigatus, as both saprophytic mould and medically important human pathogen, needs efficient mechanisms to sense
environmental signals and transduce them intracellularly to survive in its entirely different habitats. One of these signal transduction
pathways is the cAMP dependent protein kinase A (PKA) pathway. For A. fumigatus, components of this generally well conserved
signaling cascade have been characterized in detail and its significance for virulence was shown. To identify target genes of PKA, we
performed microarray analyses using a mutant strain overproducing the PKA catalytic subunit in comparison to the corresponding
wild type. Following this approach, we were able to find 282 up and 343 down regulated genes involved in different cellular processes
like carbon and nitrogen metabolism, cell cycle regulation and ribosome biogenesis. Among these genes potentially regulated by PKA,
23 transcription factors were found of which 15 were deleted and the mutant phenotypes were characterized under different cultivation
conditions. A C6 finger domain protein that shows highest upregulation of all identified transcription factors is located in a potential
secondary metabolite gene cluster. Because a gene deletion resulted only in minor phenotypical changes, an overexpression mutant of
this transcription factor was created to gain deeper insights into its function.

Abstract Number: 3

Conference Year: 2012

Link to conference website: NULL

New link: NULL

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