Identification and characterization of genes responsible for biosynthesis of kojic acid from Aspergillus oryzae.

Ref ID: 18329

Author:

Hideaki Koike , Junichiro Marui ,
1 1
Noriko Yamane ,Sumiko Ohashi , Yasunobu Terabayashi ,Motoaki Sano , Shinichi Ohashi , Eiji Ohshima , Kuniharu Tachibana , Yoshitaka Higa , Marie
Nishimura , and Masayuki Machida

Author address:

Natl. Inst. Adv. Indus. Sci. Tech. (AIST), Kanazawa Inst. Tech., Sansho Seiyaku Co., Ltd., Natl. Inst. Agrobiol.

Full conference title:

Asperfest 8

Abstract:

Aspergillus oryzae is one of the most important organisms in Japanese fermented food industry. Kojic acid is produced in large amounts by Aspergillus
oryzae as a secondary metabolite and is used in the cosmetic industry. Glucose can be converted to kojic acid, perhaps by only a few steps, but no genes
for the conversion have thus far been revealed. Using a DNA microarray, gene expression profiles under three pairs of conditions, significantly affecting
kojic acid production, were compared. All genes were ranked using an index parameter reflecting both high amounts of transcription and a high induction
ratio under producing conditions. After disruption of nine candidate genes selected from the top of the list, two genes of unknown function were found
to be responsible to kojic acid biosynthesis, one having an oxidoreductase motif and the other a transporter motif. These two genes are closely associated
in the genome, showing typical characteristics of genes involved in secondary metabolism. Regulatory mechanism of the gene cluster will be discussed.

Abstract Number: 7)

Conference Year: 2011

Link to conference website: NULL

New link: NULL

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