Heterologous expression of fungal polyketide synthases.

Ref ID: 15877

Author:

Dorte K. Holm, Bjarne G. Hansen, Thomas O. Larsen, Uffe H. Mortensen

Author address:

Mortensen Technical
University of Denmark, Department of Systems Biology, Center for Microbial Biotechnology, Denmark.

Full conference title:

26th Fungal Genetics Conference

Date: 15 March 2014

Abstract:

Polyketides are a group of secondary metabolites that comprise molecules of very distinct structures and biological activities. In fungi, polyketides are
synthesised via a multi-domain enzyme called a type I iterative polyketide synthase (PKS). As many polyketides are of medical or industrial use, various
approaches have been addressed to link genes to known polyketide products e.g. deletion of genes encoding PKSs, overexpressing transcription factors,
or using wide domain activators. Although these attempts have substantially increased our insights into polyketide biosynthesis, much is still to be learned
especially in fungi where the full genome sequence is known, but where no efficient genetic tools exist. Based on a gene expression platform developed
in our laboratory for heterologous or ectopic expression of genes in A. nidulans, we present an efficient approach for investigating polyketide synthesis
in filamentous fungi. As a test case, 37 putative and known PKS encoding genes from the industrially relevant fungus Aspergillus niger were individually
expressed in A. nidulans using A. nidulans promoters and terminators to ensure efficient transcription. This approach proved robust, fast, and efficient,
and it is highly suitable for automating the generation of expression constructs for expression in A. nidulans.

Abstract Number: NULL

Conference Year: 2011

Link to conference website: NULL

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