Heterologous expression in Aspergillus niger – a case study.

Ref ID: 18397

Author:

Nada Kraševec , Irena Lukanèiè , Tatjana Milunović , Marija An ur Lasnik , Mojca
Benèina , Vladka Gaberc Porekar , Radovan Komel

Author address:

National Institute of Chemistry, POB 660, SI-1001 Ljubljana, Slovenia. Lek Pharmaceuticals d.d.,
a Sandoz company, Verovškova 57, SI-1000 Ljubljana, Slovenia. nada.krasevec@ki.si.

Full conference title:

Asperfest 8

Abstract:

Filamentous fungi are convenient for the commercial production of heterologous proteins because oftheir natural ability to secrete high amounts of variety
of proteins. The species of the genus Aspergillus, which have obtained the GRAS status, are being used extensively for large scale production of enzymes.
Although today most of the therapeutic proteins are produced in mammalian cell cultures or prokaryotic systems, depending on the nature and molecular
weight of the protein, fungi still hold promises as an alternative expression system. To explore their potential, expression of model therapeutic protein,
human granulocyte colony stimulating factor (GCSF) was studied in the filamentous fungus Aspergillus niger. GCSF has an important role in myeloid
lineage of haematopoiesis. The natural human GCSF is O-glycosylated protein which exists in a shorter and more active form of 174 amino acids and
a longer one with 3 additional amino acids. Molecule contains one free cysteine residue and two disulfide bonds, which stabilize a functional 4 alfa-helix
tertiary structure. Hereby, we report the secretion and isolation of processed, folded and biologically active GCSF in filamentous fungi A. niger.

Abstract Number: 76)

Conference Year: 2011

Link to conference website: NULL

New link: NULL

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