Growing clinical filamentous fungi species diversity after implementing routine matrix-assisted laser desorption/ionisation-time of flight mass spectrometry (MALDI-TOF MS) identification

Ref ID: 19231

Author:

S. Ranque*, A.-C. Normand, C. Cassagne, M. Hendrickx, R. Piarroux

Author address:

Marseille, FR; Brussels, BE

Full conference title:

23rd European Congress of Clinical Microbiology and
Infectious Diseases

Date: 27 April 2014

Abstract:

Objective: MALDI-TOF Mass Spectrometry (MS)-based identification of clinical filamentous fungi has been implemented in 2011 in the routine identification procedure of clinical filamentous fungi at the Mycology laboratory of the University hospital of Marseille. This study aimed to compare the identifications results over a six month period in 2012 with those obtained before the use of MS.
Methods: In 2012, all filamentous fungi isolated in the routine activity of the mycology laboratory were identified using both macro- and microscopic morphological criteria and MS on a Microflex® instrument with the MALDIBiotyper® software (Bruker Daltonics) using an in-house reference spectra database developed in collaboration with the BCCM/IHEM collection. Discrepancies between MS and morphological identification were resolved by ITS2 and D1-D2 sequencing. Results from 2012 were compared to those of a representative sample of isolates identified in the same laboratory in 2009 and 2010, before using MS.
Results: Over six months routine activity in 2012, 568 isolates were identified in the laboratory. They belonged to 90 distinct taxa, mainly grouped in Aspergillus (28%); Penicillium (20%); filamentous Basidiomycetes (12%); Fusarium (10%); Pleosporales (8%); Pseudallescheria/Scedosporium (4%); Trichoderma (3%) and others (<3% each). 81 (14%) were identified by DNA sequencing. All but three (0.5%) Penicillium sp. were identified to species in 2012. In comparison, before the use of MS 521 isolates identified consisted of 19 distinct taxa of which 10 were only identified to genus. Conclusion: The use of MALDI-TOF MS with an adequate reference spectra library critically enhanced the routine identification of filamentous fungi in the clinical laboratory, as evidenced by the 10 fold increase in the diversity of taxa identified to species. It brings to the bench a mycological identification accuracy level that was before restricted to a few reference laboratories.

Abstract Number: P1725

Conference Year: 2013

Link to conference website: http://registration.akm.ch/einsicht.php?XNABSTRACT_ID=165449&XNSPRACHE_ID=2&XNKONGRESS_ID=180&XNMASK

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