Gold nanoparticles in Aspergillus nidulans hyphae: can we study real-time physiology?

Ref ID: 15852

Author:

Susan Kaminskyj (a), Martin Prusinkiewicz (a), Fatemeh
Faraz-Khorasani (b), Merrill Isenor (b), Kathleen Gough (b)

Author address:

Dept Biology, Univ Saskatchewan, Canada; b) Dept Chemistry, Univ Manitoba, Canada

Full conference title:

26th Fungal Genetics Conference

Date: 15 March 2014

Abstract:

High spatial resolution methods to analyze biochemical composition of individual hyphae can assess cell physiology during growth in optimal or stressed
conditions. Whole colony methods like GC-MS cannot capture all the details of physiology and organism-environment interaction. In addition to Fourier
transform infrared (FTIR) spectromicroscopy, surface-enhanced Raman spectroscopy (SERS) can provide biochemical characterization of components
that are in contact with gold that has been nano-patterned (Klarite substrate) or has formed nano-particles (AuNPs). SERS can potentially be used to
examine biochemical processes in living cells. We have grown AuNPs within and on the surface of Aspergillus nidulans hyphae, and documented their
distribution and composition using transmission electron microscopy and scanning transmission x-ray microscopy. Most AuNPs were associated with
hyphal walls, both in the cytoplasm and on the wall surface. AuNPs grown in cultures treated for 2 h with 1 mM Au3+ appeared to be optimal for
generating SERS activity. The AuNP spectra were more complex than most SERS spectra from A. nidulans hyphae grown on a Klarite substrate.
Interpreting SERS spectra will be challenging, and will require validation for the diversity of molecules present on the wall and in the peripheral
cytoplasm. To date we have proof in principle that it will be possible to generate SERS spectra in living hyphae.

Abstract Number: NULL

Conference Year: 2011

Link to conference website: NULL

New link: NULL

Conference abstracts, posters & presentations

Showing 10 posts of 17325 posts found.

  • Title

    Author

    Year

    Number

    Poster