Gle1 translocates from the nuclear pore complex to the nuclear membrane during mitosis in Aspergillus nidulans

Ref ID: 18370

Author:

Mahesh Chemudupati, Aysha
Osmani and Stephen A. Osmani

Author address:

Department of Molecular Genetics, The Ohio State University, Columbus, OH

Full conference title:

Asperfest 8

Abstract:

During mitosis in Aspergillus nidulans, a subset of nuclear pore complex proteins (Nups) disperses from the core pore structure. This subset of Nups
includes all budding yeast orthologs classified as peripheral Nups, with one exception. The predicted peripheral Nup An-Gle1 is unique in that it remains
at the nuclear envelope (NE) during mitosis, an attribute typical of core Nups (Osmani et al. Mol. Biol. Cell, 17, 4946-4961, 2006). Additionally, GFP
labeled An- Gle1 is distinctly located at the nuclear membrane surrounding the nucleolus during mitosis (Ukil et al. Mol. Biol. Cell, 20, 2132-2145, 2009).
Affinity purification of all known Nups in A. nidulans identified a protein that co- purified with An-Gle1. This protein, AN0162, has a predicted Cterminal transmembrane domain. Endogenously GFP- tagged full-length AN0162 locates to the nuclear membrane throughout mitosis in a manner
identical to An-Gle1 as does the GFP-tagged C-terminal half of the protein. Further analysis showed that AN0162 is responsible for tethering An-Gle1
to the NE specifically during mitosis, but not during interphase. This is the first known instance of a protein targeted to the NE by two different
mechanisms. An-Gle1 is targeted to NPCs during interphase, but during mitosis An-Gle1 is targeted to the nuclear membrane by associating with AN0162.
Our findings thus agree with the categorization of An-Gle1 as a peripheral Nup. This expands our knowledge of how proteins can be targeted to the NE
in a regulated cell-cycle dependent manner. Supported by National Institutes of Health grant GM042564

Abstract Number: 48)

Conference Year: 2011

Link to conference website: NULL

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