Genomics of Aspergillus oryzae and effective utilization of large scale genomic information.

Ref ID: 18530

Author:

Masayuki Machida,
Hideaki Koike,
Yoshinori Koyama,
Hiroko Hagiwara,
Tomoko Ishii,
Itaru Takeda,
Myco
Umemura,
Koichi Tamano,
Isao Kojima,
AIST fungal genome

Author address:

AIST/TUAT  
AIST

Full conference title:

11 th European Conference on Fungal Genetics

Abstract:

Recently, we have determined the genes responsible to the biosynthesis of kojic acid from A. oryzae, which is used
as a cosmetic whitening agent. Transcriptome analysis and successive gene disruption approach has been
successfully applied to the gene identification. We found three genes encoding oxidereductase, transporter and
transcription factor, which are localized on the non8208;syntenic block. It took, however, more than one year for the
identification because the target gene prediction was inaccurate and disruption of roughly 20 genes was required
before finding the genes. To accelerate identification of the genes responsible to biosynthesis of novel
metabolites, we have prepared our pipelines from genome sequencing to gene detection, which includes DNA
sequencer, DNA microarray and LC/MS.
We have improved the performance of the de novo assembling pipeline for ABI SOLiD, and have optimized it for
sequencing of microorganism genomes. Our pipeline generates scaffolds longer than 1 Mb, covering 99% of
approximately 40 Mb genome with roughly 100 or less scaffolds in general. Resulted sequences are subjected to
annotation, comparative analysis and the highly accurate gene prediction that we have developed. We have found
that our pipeline detected the genes for secondary metabolites that have been already known. We are now
evaluating the performance in accuracy by disruption and overexpression of some novel genes that have been
predicted by the pipeline.

Abstract Number: PR8.45

Conference Year: 2012

Link to conference website: http://www.ecfg.info/images/Abstract_Book_Electronic.pdf

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