Genome wide polyketide synthase gene deletion library in Aspergillus nidulans.

Ref ID: 15876

Author:

Uffe H. Mortensen, Michael L. Nielsen, Jakob B. Nielsen,
Christian Rank, Marie L. Klejnstrup, Dorte K. Holm, Katrine H. Brogaard, Bjarne G. Hansen, Jens C. Frisvad, Thomas O. Larsen

Author address:

Center for Microbial
Biotechnology, Department of Systems Biology, Technical University of Denmark, Sí¸ltofts Plads B221/B223, 2800 Kgs. Lyngby, Denmark

Full conference title:

26th Fungal Genetics Conference

Date: 15 March 2014

Abstract:

Filamentous fungi produce a wealth of secondary metabolites that dramatically influence human life as they comprise both mycotoxins and
pharmaceuticals. To forward the understanding of fungal secondary metabolism we have adopted a multidisciplinary strategy based on fungal ecology,
analytical chemistry and molecular biology. Here we present an analysis of a library containing individual deletions of all known and putative PKS genes
in the model fungus Aspergillus nidulans. The library has been challenged on a number of different media to uncover new links between genes and
polyketide products. At our conditions, we detect several of the known products of A. nidulans including the PKS gene responsible for production of
austinol. The validity of this conclusion is ensured by further mutagenesis including site directed mutagenesis of the locus as well as ectopic expression
of the gene to indentify the first intermediate in austinol production. Based on our findings we call the gene ausA. Moreover, our results demonstrate
several examples of crosstalk between different pathways in polyketide synthesis. To this end, it is important to stress that by investigating a genome wide
deletion library at different conditions the chance of mis-assigning genetic links to products due to such crosstalk is dramatically reduced. Together the
results and conclusions presented constitute our first step towards a systems understanding of the secondary metabolism of A. nidulans.

Abstract Number: NULL

Conference Year: 2011

Link to conference website: NULL

New link: NULL

Conference abstracts, posters & presentations

Showing 10 posts of 17325 posts found.

  • Title

    Author

    Year

    Number

    Poster