Genome wide insights into the targets and mechanism of function of LAE1 in Trichoderma reesei  

Ref ID: 18463

Author:

Razieh Karimi Aghcheh, Pallavi A. Phatale, Kristina M. Smith, Scott E. Baker, JinWoo Bok, Bernhard Seiboth, Nancy P.Keller, Michael Freitag, Christian P. Kubicek

Author address:

TU Wien, Institute of Chemical Engineering, Division Applied Biochemistry and Gene Technology,  
Oregon State
University, Department of Biochemistry and Biophysics, USA.
Fungal Biotechnology Team, Chemical and Biological
Process Development Group

Full conference title:

11 th European Conference on Fungal Genetics

Abstract:

In Aspergillus spp., the laeA (loss of aflR expression) gene acts as global regulator of secondary metabolism. We
found that the T. reesei orthologue LAE1 regulates cellulase and hemicellulase gene expression (Seiboth, Karimi et
al. unpublished data). In order to identify all targets of LAE1 in T. reesei, we analysed the transcriptome of lae1
deletion (Dlae1) and LAE1 overexpressing (OE) strains during growth on lactose. 1186 (13 % of the   total 9143)
genes of T. reesei were affected more than 28208;fold in the Dlae1 strain, and 701 in the OE strain, and almost all of the
latter (696) were also significantly expressed in the former strain. Amino acid permeases, ankyrins, and G8208;protein
coupled receptors were most strongly downregulated in Dlae1 and upregulated in OE. Expression of known (PKS,
NRPS) or predicted (monooxygenases, dioxgenases, multicopper oxidases) enzymes involved in secondary
metabolite production was also affected, with roughly equal numbers of genes being up8208;  and downregulated.
Approximately 15 % of the genes presumably regulated by LAE1 occurred in 40 discrete clusters in the T. reesei
genome. ChIP8208;seq analyses suggest that LAE1 does not directly alter the balance of an active (H3K4me2) and
silencing (H3K9me3) histone modification under our conditions. Conidiation was strongly reduced in the delta8208;lae1
strain, both in light and in darkness, and about one third of conidiation genes were affected in the Dlae1 strain,
indicating a major effect of LAE1 on T. reesei sporulation. Our data expand the current knowledge of LAE1 function
and point to a major involvement in conidiation in T. reesei.

Abstract Number: PR1.48

Conference Year: 2012

Link to conference website: http://www.ecfg.info/images/Abstract_Book_Electronic.pdf

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