Genetic And Molecular Characterization Of The Penicillium chrysogenum PcrsmA Gene, Encoding A Homologue Of The Aspergillus nidulans bZIP Transcription Factor RsmA

Ref ID: 18520

Author:

Kordula Becker, Sandra Bloemendal, Ulrich Kück

Author address:

Christian Doppler Labor für “žBiotechnologie der Pilze”œ, Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr-
Universität Bochum, D-44780 Bochum  

Full conference title:

11 th European Conference on Fungal Genetics

Abstract:

Penicillium chrysogenum is the major industrial producer of the íŸ8208;lactam antibiotic pencillin. The regulation of
cellular processes like secondary metabolism as well as morphogenesis and development in this filamentous
fungus is controlled by components of the so called velvet8208;like complex which was first described in Aspergillus
nidulans. Previous studies identified rsmA (remediator of secondary metabolism) in A. nidulans which after
overexpression was shown to remediate secondary metabolism defects in knockout strains of the velvet
components LaeA and VeA. Hence, it is believed that the putative bZIP transcription factor RsmA may act as a
positive regulator of many secondary metabolite pathways. Bioinformatics confirmed the existence of many
known and putative bZIP proteins from several fungi such as P. chrysogenum and higher eukaryotes which show
high homology to RsmA from A. nidulans.
Until now, little is known about the biosynthetic regulation of the íŸ8208;lactam antibiotic penicillin. Thus, further
investigation of putative regulators of secondary metabolism such as PcRsmA, the P. chrysogenum homologue to
RsmA is necessary to extend the current knowledge of the regulatory network controlling both penicillin
biosynthesis and morphogenesis in P. chrysogenum. A 8710;Pcku70 strain as recipient for homologous recombination
together with the FLP/FRT recombination system were used to generate a marker8208;free  8710;PcrsmA strain for
functional and morphological characterization of PcRsmA. Our results will support deciphering of regulatory
networks related to the velvet8208;like complex in P. chrysogenum.

Abstract Number: PR8.21

Conference Year: 2012

Link to conference website: http://www.ecfg.info/images/Abstract_Book_Electronic.pdf

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