EZ Menkem1*, RMT Kouipou1, CDJ Mbouna1, FF Boyom1

Ref ID: 19486

Author:

Anti -fungal activity of Uvariodendron calop hyllum
fractions

Author address:

1Department of Biochemistry, University of Yaounde 1, Yaounde, Cameroon
Purpose:

Full conference title:

6th Advances Against Aspergillosis 2014

Abstract:

Purpose:
Aspergillosis is a spectrum of diseases of humans and animals caused by members of the genus
Aspergillus. Aspergillus sp. organisms are capable of living both a saprophytic and parasitic way
of life and susceptible hosts have numerous opportunities to contact this agent. Aspergillosis
and Candida species are opportunistic pathogens that cause superficial and systemic candidiasis
particularly in immunocompromised patients. Resistance to many clinically used antifungal agents
has created a need to identify new compounds and develop drugs for therapeutic use. Therefore, the
main objective was to carry out bioguided fractionation of the ethanolic and aqueous extracts of the
leaves, twigs, stem, stembark and trunck of Uvariodendron calophyllum.
Methods:
The leaves were macerated in ethanol and water, their yields of extraction were calculated. The
broth micro dilution and agar dilution method was used for antifungal susceptibility testing of yeasts
(Candida albicans, Candida glabrata, Candida parapsilosis, Cryptococcus neoformans isolates
and four candida albicans strains ATCC L26, ATCC 12C, ATCC P37039, ATCC P37037) and
moulds (Aspergillus fumigatus, Aspergillus niger, and Aspergillus flavus). Phytochemical screening
on the crude extracts was done. Then, bioguided fractionation was done on the crude extracts using
Hexane, Methylene Chloride and Methanol. Column chromatography was done on the methylene
chloride fraction of the leaf ethanolic extract using different solvent systems.
Results:
The yields of extraction ranged from 1.125% to 6.77% for the aqueous extract and from 3.57%
to 10.67% for the ethanol extracts. The phytochemical screening showed the presence of
phenols, tannins, flavonoids and glycosides in both extracts. The MIC ranged from 0.63±0.27 to
2.19±1.43 mg/mL for the ethanol extracts, from 1.88±0.00 to 7.50±0.00mg/mL for the aqueous
extract on the Yeasts microorganisms. The various MIC ranged from 22 -36mg/ml on A. Flavus,
23-35mg/ml on A. niger and 23-36mg/ml on A. fumigatus. The most active was obtained for
U. calophyllum twig extract (22 mg/ml) on A. flavus strain.The MIC of amphotericine B and
griseofulvin were greater than 2mg/ml. 35 fractions were obtained after partition and their MIC
results ranged from 1.25±0.00 to ≥ 10±0.00 mg/mL on the tested microorganisms. Nineteen fractions
were obtained and labeled fraction A to fraction S with MIC varying from 0.002 to ≥ 2mg/mL with
fraction S having the best activity.
Conclusion:
The crude ethanol, aqueous extracts and fractions of Uvariodendron calophyllum could be potential
sources of compounds with anti-fungal activity.
Key words:
Bioguided fractionation, Anti-fungal activity, Uvariondendron calophyllum, phytochemicals

Abstract Number: 14

Conference Year: 2014

Link to conference website: http://www.AAA2014.org

New link: NULL

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