Extracellular enzymes from Trichoderma harzianum, Aspergillus terreus and AM fungus

Ref ID: 18535

Author:

Bakhtiyor Umarov, Khurshede Khamidova

Author address:

Institute of Microbiology, Uzbekistan  

Full conference title:

11 th European Conference on Fungal Genetics

Abstract:

Cellulotic enzymes consistitute form the group well described biologically important enzymes,The enzyme
cellulase, a multi enzyme complex made up of several proteins, catalyses theconversion of cellulose to glucose in
an enzymatic hydrolysis. Which catalyze the transfer of the glycosyl group between oxygen nucleophiles.
important role this enzymes in the biology is  include  degradation celluloses of the biomass fungi and bacteria,
degradation of glycolipids in mammalian lysosomes, and the cleavage of glycosylated flavonoids in plants.  Fungus  
Trichoderma and Aspergillus have biotechnologicaly importance, since they are a producer extracellular enzyme.  
We  studies from the new strains  selection high cellulotic activities and using them in the  biotechnical  studies.
Studing   extracellular enzymes   from 38208;fungi, Aspergillus terreus,   Trichoderma harzianum and Mucorrhiza
conducted in minimum ambience, containing wheat bran as single source of the carbon.  Enzyme activities were
assayed spectrophotometrically by using Samogy8208;Nelson. Temperature and pH optimum of some purified enzymes
were determined also. The isolation and purification cellulotic enzymes we are used the ion exchange
chromatography on DEAE TOYOPEARL 650 M gel   in the gradient 0,51052; NaCl. The test strains of each species .,
Aspergillus terreus, Trichoderma harzianum and AM fungi were analyzed on the basis of extent of hydrolyzing
ability. Cellulose hydrolysis of all three strains was immensely affected by varying pH and medium. The results
indicated statistically significant interaction in all correlating factors of strain, growth medium and pH level

Abstract Number: PR8.62

Conference Year: 2012

Link to conference website: http://www.ecfg.info/images/Abstract_Book_Electronic.pdf

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