Expression Response Of Aspergillus oryzae To Different Nitrogen Sources In Batch Cultivations

Ref ID: 18529

Author:

Christoph Hubert Knuf, Intawat Nookaew, Jens Nielsen 

Author address:

Systems and Synthetic Biology Group, Department of Chemical and Biological Engineering, Chalmers University of
Technology

Full conference title:

11 th European Conference on Fungal Genetics

Abstract:

The filamentous fungus Aspergillus oryzae is widely used as a microbial cell factory for large scale heterologous
protein production. A. oryzae is also known as a natural organic acid producer. However, the metabolism and
regulation of organic acid production in A. oryzae are poorly characterized. Furthermore, the media composition
has a major impact on the performance of this organism and the economic feasibility of an industrial fermentation
process. We therefore evaluated the global expression response towards and the utilization of different nitrogen
sources by A. oryzae in batch fermentations.
In this study, we aim for a deep investigation of the cellular mechanisms of the utilization of different nitrogen
sources. Firstly, we performed batch cultivations with two strains (NRRL3488 and DSM1862) on defined and
complex nitrogen sources using di8208;ammonium sulphate and peptone, respectively. In addition, transcriptome
analysis was performed on samples from these fermentations to analyze the gene expression under exponential
growth conditions (mid8208;exponential phase; 6h) and in nitrogen starvation (stationary phase; 30h) to further
identify key8208;players in the metabolism and regulation of gene expression.
Cluster analysis revealed a conserved response of both strains and helped to identify regulatory sequences among
the co8208;expressed genes. Furthermore the expression data pointed towards malic acid production as a respond to
nitrogen starvation stress.

Abstract Number: PR8.42

Conference Year: 2012

Link to conference website: http://www.ecfg.info/images/Abstract_Book_Electronic.pdf

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