Exploratory survey for potential transposable elements in Aspergillus oryzae by a stress-fluctuation cDNA browser

Ref ID: 18348

Author:

Hironobu Ogasawara , Saori
Takahashi , and Katsuya Gomi

Author address:

AkitaRes.Inst. Food and Brewing, Akita, Japan. Graduate School of Agricultural Science, Tohoku University, Sendai,
Japan. E-mail:hironobu@arif.pref.akita.jp

Full conference title:

Asperfest 8

Abstract:

An active DNA transposon Crawler isolated from the genome of industrially important fungus Aspergillus oryzae transposes under extreme stress
conditions [1]. The mRNA analysis of Crawler in the conidiospores revealed that cryptic splicing and premature polyadenylation of the mRNA occurred
under
4
the normal culture condition. The increasing in mature mRNA molecules was caused by stress treatment of CuSO or heat shock, which could
stimulate the transposition events allowing the full-length and active transposase to be produced. @In this study, we carried out direct high-throughput
paired-end RNA @sequencing to construct a stress-fluctuation cDNA browser with DOGAN-DB to survey exogenous or transposon-like genes such as
Crawler
4
in A. oryzae. With comparison of expression pattern under extreme stress condition (CuSO ) to the normal condition, several novel transcripts
with open reading frames were identified in intergenic regions, where none of genes have been annotated in DOGAN-DB. Full length of DNA sequences
encoding transposable elements were frequently identified. Among them, a novel transposable element homologous to Tan1 from A. niger was identified
and tentatively designated AoTan1 that shows multiple characteristics of class II transposon. The elements are present as multiple copies in the genome
of the RIB40 strain, suggesting that AoTan1 is also expected to show a transposition activity. 1)H. Ogasawara et al. Fungal Genet. Biol. , 46, 441-449
(2009)

Abstract Number: 26)

Conference Year: 2011

Link to conference website: NULL

New link: NULL

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