Evaluation of Vitek 2 system for identification of most clinically important Candida species

Ref ID: 17776

Author:

M.E. Ochiuzzi, I. Maldonado, L. Guelfand, A. Arechavala,
R. Soloaga*

Author address:

(Buenos Aires, AR)

Full conference title:

22nd European Congress of Clinical Microbiology and Infectious Diseases

Abstract:

Objective: The species of Candida are the major cause of invasive
fungal infections in hospitalized patients. Identification of yeasts to the
species level has become a priority, not only for the increasing number
of non-Candida albicans isolates but also for the differential
susceptibilities to antifungal agents, being important at this point for
the therapeutic management. VITEK 2 (BioMe’rieux, Inc, Hazelwood,
MO, USA) is a fully automated microbiology instrument that allows the
identification of microorganisms, including yeasts. This study utilized
ID-YST card using a colorimetric system which include 47 biochemical
tests (a variety of carbohydrate and organic acids assimilation tests,
oxidase and arilamydase enzyme detection). The aim of our study was
to evaluate the YST card of VITEK 2 System for the identification of
the most frequent yeasts in clinical microbiology laboratory.
Methods: A total of 168 strains of Candida were studied: C. albicans
(n = 26), C. tropicalis (n = 29), C. glabrata (n = 28), C. parapsilosis
(n = 32), C. krusei (n = 26), C. guilliermondii (n = 20), C. dubliniensis
(n = 7); 164 isolates came from clinical samples of hospitalized and
ambulatory patients received in hospitals included in the Mycology Net
of Buenos Aires, Argentina. Four pattern strains: C. parapsilosis ATCC
22019, C. krusei ATCC 6258, C. glabrata ATCC 90030 and C.
albicans ATCC 64548 were included. The strains were transferred from
storage at )70C onto Sabouraud agar and subcultured after 24 hour on
Sabouraud agar. All strains were tested with the ID-YST card and API
20C or API 32C system (BioMe’rieux, Marcy L’Etoile, France),
according the manufacturer’s instructions and the results were
compared. The API was considered the reference standard for
accuracy of identification.
Results: The percentage of concordance between API and VITEK 2
Yeast card was 98.3%. There were three misidentifications: one C.
parapsilosis, one C. krusei and one C. tropicalis. The average time of
identification was 18.2 hour (range 18.0-18.5 hour).
Conclusions: According to the results of our study VITEK 2 System is
a reliable, simple and effective method for the identification of the most
frequent Candida spp and besides, reduced the times for identification
from 48 to 18 hour.

Abstract Number: NULL

Conference Year: 2012

Link to conference website: NULL

New link: NULL

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