Evaluation of anti-Aspergillus activity of bioactive secondary metabolites from endophytic fungus Pestalotiopsis microspora IP-310 isolated from leaves of medicinal plant Berberis lyceum

IP Perveen1, SA Ahmed2, SS Sehar2, IN Naz2

Author address: 

1Microbiology, Shaheed Zulfiqar Ali Bhutto Medical University, Islamabad, Pakistan 2Microbiology, Quaid-i-Azam University, Islamabad, Pakistan


Purpose: To evaluate the anti-Aspergillus activity of endophytes isolated from a medicinal plant Berberis lyceum

Methods: Six endophytic fungi were isolated from the leaves of medicinal plant Berberis lycium and the isolated species were identified phenotypically and genotypically. The fungal cultures were further grown and the culture was extracted by two organic solvents methanol and ethyl acetate. The screening was carried out using the disc diffusion method and microbroth dilution assay against three fungal strains of Aspergillus i.e. Aspergillus fumigatus, Aspergillus flavus and Aspergillus niger.

Results: Minimum inhibitory concentration of extracts was determined by microtiter dilution assay. Ethyl acetate extract of Pestalotiopsis microspore IP-310showed maximum activity against A. fumigatus (0.65 mg/ml). The ethyl acetate extracts Pestalotiopsis microspore IP-310 was further evaluated for its anti-Aspergillus potential using disc diffusion assay. Maximum zone of inhibition found was 14.5 ± 0.5 mm against A. fumigatus.

Conclusion: These results indicate that endophytic fungi P. microspore isolated from medicinal plant Berberis lyceum could be a potential source of bioactive compounds against Aspergillus Sp. and may find potential use in pharmaceutical industry.


Full conference title: 

The 8th Advances Against Aspergillus, Lisbon Conference Center, Lisbon, Portugal
    • AAA 8th (2018)