Establishment of tools for the analysis of vesicle traffic in Aspergillus nidulans

Ref ID: 18458

Author:

Nathalie Grün, Reinhard Fischer

Author address:

Karlsruhe Institute of Technology, Germany

Full conference title:

11 th European Conference on Fungal Genetics

Abstract:

In order to allow the extreme polar hyphal growth of filamentous fungi membrane lipids and cell wall synthesizing
enzymes are needed constantly in the growth area. This is achieved by a continuous flow of secretory vesicles from
the Golgi apparatus to the growing tip. For efficient growth, cell end markers and enzymes anchored in the plasma
membrane are removed from the plasma membrane subapically by endocytosis. Thus filamentous growth
depends on different populations of vesicles, which may use different routes for transportation. In order to
investigate the dynamics and role of different vesicles, we studied the localization of different proteins of both the
endocytotic and exocytotic pathway. SynA, a v8208;SNARE protein and clathrin heavy8208;chain are components of both
pathways. In addition, three putative secreted proteins,  β 8208;glucosidase with a signal peptide (BglA) and two
proteins without a signal peptide, the glucan synthase regulatory protein (GlsA) and the chitin synthase (ChsB),
were studied.
By co8208;localization with fluorescently labeled microtubules we showed transport along these filaments. SynA, BglA,
GlsA und ChsB were transported by conventional kinesin towards the Spitzenkörper, were they accumulated. After
deletion of the kinesin8208;1 the anterograde transport was taken over by other motor proteins like kinesin8208;3.
At the tip the secretory vesicles containing SynA fused with the plasma membrane and were internalized at the
subapical region. The internalized vesicles called early endosomes were transported by dynein.

Abstract Number: PR1.39

Conference Year: 2012

Link to conference website: http://www.ecfg.info/images/Abstract_Book_Electronic.pdf

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