Epidemiology of airway colonisation by Rasamsonia argillacea during cystic fibrosis

Ref ID: 19241


O. Matray, S. Giraud, G. Gargala, J. Bouchara, M.E. Bougnoux, L. Favennec*

Author address:

Rouen, Angers, Paris, FR

Full conference title:

23rd European Congress of Clinical Microbiology and
Infectious Diseases

Date: 27 April 2014


Objective: Species of the Rasamsonia argillacea complex are presently considered as an emerging opportunistic agent in cystic fibrosis (CF) patients. Although the clinical significance of their isolation from respiratory secretions in CF still remain a matter of debate, it is clearly established now that these fungi may disseminate in case of immunodeficiency. The aim of this work was to evaluate the automated typing system DiversiLab to investigate the molecular epidemiology of the airway colonization by R. argillacea
Method: 55 multiple and sequential isolates of R. argillacea collected from 15 CF patients from Rouen, Giens and Angers (France) were analyzed using the recently described automated typing system DiversiLab (bioMí©rieux) based on PCR amplification of repetitive sequences. Two different rep-PCR kits were compared: the DiversiLab Aspergillus kit which uses specific primers designed for Aspergillus fumigatus and the pan-fungus DiversiLab Fungal kit. The amplification products were separated by capillary electrophoresis on Agilent B2100 bioanalyzer, leading to single profiles for each isolate which were then compared using the DiversiLab software.
Results: Results obtained with both kits were comparable. Nevertheless, differentiation was easier using the DiversiLab Fungal kit. Among the 55 isolates studied, 28 different genotypes were found. Sequential isolates from some patients exhibit different genotypes whereas the same genotype was isolated from epidemiologically unrelated patients from different cities.
Moreover, 6 out if 15 patients were chronically colonized by the same genotypes.
Conclusion: The automated typing system DiversiLab proved to be an easy and efficient method to investigate the molecular epidemiology of the airway colonization by R. argillacea in CF. Moreover, our results confirm the capacity of the different species from the R. argillacea complex to chronically colonize the airways of CF patients despite the repeated antifungal cures.

Abstract Number: P2428

Conference Year: 2013

Link to conference website: http://registration.akm.ch/einsicht.php?XNABSTRACT_ID=167129&XNSPRACHE_ID=2&XNKONGRESS_ID=180&XNMASK

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