Effect of 946;-glucan supplementation on the growth and cell wall structure of Aspergillus spp

Ref ID: 19608

Author:

K Ishibashi1*, S Utsugi1, T Toyoshima1, N Miura1, Y Adachi1, N Ohno1

Author address:

1Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of
Pharmacy and Life Sciences, Tokyo, Japan

Full conference title:

6th Advances Against Aspergillosis 2014

Abstract:

Purpose:
β -Glucan is one of the main component of fungal cell wall polysaccharides. It was detected in
the culture supernatants of Aspergillus and Candida. β -Glucan has been reported to show various
biological activities such as production of the mediators of the inflammation in vivo and in vitro.
However, only few studies have examined the effect of β -glucan on the fungal cell itself. In this
study, we investigated the effect of β -glucan supplementation on the growth and cell wall structure
of Aspergillus spp.
Methods:
Aspergillus fumigatus was cultured in synthetic media supplemented with β -glucan (curdlan and
laminarin). The morphological changes in Aspergillus induced by curdlan and laminarin were
observed under a the microscope and compared. The sodium hypochlorite (NaClO) oxidized cells
of the fungus body in media supplemented with or without β -glucan were prepared and the structure
of these cells analyzed by carbon-nuclear magnetic resonance (C-NMR).
Results:
In β -glucan added medium, the promotion of the growth assessed by increase in the turbidity of the
medium was determind and compared with that of the control medium on day 1. Hyphal growth was
promoted in liquid and solid-cultures with addition of β -1,3-glucan. Glucose and dextran did not
induce growth. In the control medium, α -1,3-glucan was the main component, but in the β -glucan
added medium, the peak ratio of β -1,3-glucan increased. We compared the reactive oxygen species
(ROS) production by macrophages that were stimulated in the A. fumigatus dried cells with β -glucan
addition. A. fumigatus dried cells in β -glucan added medium induced higher ROS production than
A. fumigatus dried cells in the control medium.
Conclusion:
The addition of β -glucan in the culture medium influenced the growth of the fungi and induced
qualitative changes in the cell wall structure. Because β -glucan is detected in the mycology-culture
supernatant, the relativity between these changes and the pathogenicity of Aspergillus spp has an
interest.

Abstract Number: 133

Conference Year: 2014

Link to conference website: http://www.AAA2014.org

New link: NULL

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