Antimicrobial peptides have merited renewed interest due to their potential to disperse microbial biofilms and cancer therapeutic properties. Inhibition strategies based on the planktonic bacterial physiology have been known to have restricted efficacy on the control of biofilm communities. This problem can be intensified by the emergence of increasingly resistant pathogenic strains. In the present study, we have isolated and characterized an extracellular antibacterial peptide designated as MFAP9 from a marine derived fungus Aspergillus fumigatus BTMF9 using DEAE Sepharose-based column chromatography. MFAP9 showed a single band of 3 kDa on SDS-PAGE and the homogeneity showed retention time of 32.5 min in RP-HPLC. In vitro antibiofilm and antiproliferative properties of MFAP9 were tested which exhibited superlative inhibition against Gram-positive bacteria and lung carcinoma cell line A549. SEM analysis of the bacterial biofilm inhibition after MFAP9 treatment showed complete disruption of biofilm with rupture of individual cells as well. In vitro cancer cell viability upon treatment with the peptide fractions assessed by MTT assay and AO/EtBr staining proved significant cytotoxic effects on A549 cell line (IC50 - 29μg/mL). This is the first report on isolation of a peptide from marine derived Aspergillus fumigatus with a promising potential as an effective tool in medical and therapeutic applications.
Full conference title:
- MS 2017