Diversity of Emericella species from soil and in vitro efficacy against plant pathogenic fungi

Ref ID: 19336

Author:

Wonvisa Kumsorn, Onuma Piasai, Leka Manoch, Chiradej Chamsawarng, Takashi Yaguchi

Author address:

Department of Plant Pathology, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand; Department of Plant Pathology, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University,
Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand

Full conference title:

Asian Mycological Congress 2013 and the 13th International Marine and Freshwater Mycology Symposium

Date: 19 August 2014

Abstract:

Soil, cotton fibre and animal dung samples were collected from various locations. The soil plate,
soil dilution plate and alcohol treatment methods as well as the soil plate and Gochenaur8223;s glucose
ammonium nitrate agar (GAN) were used for isolation of Emericella spp. Macroscopic morphological
study of Emericella spp. were recorded as growth rate, growth pattern and colour on standard culture
media. Microscopic morphological study was examined as conidial head, conidia, ascospore and hülle
cells under light and scanning electron microscopes. Molecular analysis of the fungal strain were
employed based on the 61538;-tubulin gene and analyzed with the BLAST search. Nineteen isolates of
Emericella belonging to 6 species were found including E. corrugate, E. nidulans, E. purpurea, E.
quadrilineata, E. rugulosa and E. variecolor. Nothworthy Emericella representing new records for
Thailand are E. corrugate, E. purpurea and E. quadrilineata. In vitro antagonistic test was carried out
using 10 isolates of Emericella spp. as dual cultures on PDA against 11 species of plant pathogenic fungi
as dual culture on PDA, including Alternaria brassicicola, Curvularia lunata, Colletotrichum capsici,
Colletotrichum gloeosporioides, Fusarium oxysporum, Helminthosporium sp., Pestalotiopsis sp.,
Phytophthora palmivora, Pythium aphanidermatum, Rhizoctonia oryzae and Sclerotium rolfsii. The
result revealed that E. corrugate, E. nidulans, E. rugulosa and E. variecolor. could inhibit 45-63%
mycelium growth of Alternaria brassicicola, Curvularia lunata, Colletotrichum capsici, Colletotrichum
gloeosporioides, Fusarium oxysporum, Helminthosporium sp., Pestalotiopsis sp. and Phytophthora
palmivora, whereas all of isolates failed to inhibit mycelial growth of Pythium aphanidermatum and two
Basidiomycetes anamorphs, Rhizoctonia oryzae and Sclerotium rolfsii.

Abstract Number: P-79

Conference Year: 2013

Link to conference website: NULL

New link: NULL


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