Direct Colony PCR-RFLP (DC PCR-RFLP) for rapid identification of six medically important Candida species

Ref ID: 19624

Author:

Rajyoganandh SV*r Vijayakumar R, PremamaliniT and Anupma Jyoti Kindo

Author address:

Department of Microbiology, Sri Ramachandra Medical college and Research Institute, Sri Ramachandra University,
Porur, Chennai-600 116

Full conference title:

Society for Indian Human and Animal Mycologists 2014

Date: 10 January 2014

Abstract:

Candida sp., are the most common cause of blood stream infections caused by fungi. The epidemiology of Candida species
causing blood stream infection has been shifting towards the non-albicans strains. High mortality associated with candidemia
can be reduced by prompt, appropriate antifungal therapy. Hence, rapid identification and speciation of Candida isolates from
blood play a significant role in management of the patients. Since, conventional methods used for speciation of Candida are
laborious, time consuming and often unclear, rapid and accurate molecular techniques are required. We have standardized
Direct Colony PCR-RFLP has been standardized in our laboratory which reduces the time and workforce in DNA extraction
process. In the present study we have performed Direct Colony PCR for six medically important Candida speciestargeting
ITS1,5.8S RNAandITS4 sequence, the PCR was performed products were then digested with restriction enzyme (Mspl). 49
blood isolates were subjected to this procedure and identified. This approach not only reduces the DNA template preparation
time, but is also easy, rapid and reduces the cost of PCR.
9830;Corresponding author
E-mail:rajyoganandh@yahoo.co.in

Abstract Number: OP-005

Conference Year: 2014

Link to conference website: http://www.siham2014.com

New link: NULL

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