Diagnosis of pulmonary aspergilloma by serological Aspergillus antibody detection: comparison of a new commercial immunoblot-based test with detection by immunoprecipitation

Ref ID: 19385

Author:

A. G. Paugam,1 D. Toubas,2 E. Dannaoui,3 P.Le Pape,2
M. Cornet2 and F. Persat2

Author address:

1CHU Cochin, France; 2CHU, Reims, France and 3CHU Pompidou,
Paris, France

Full conference title:

6th Trends in Medical Mycology 2013

Date: 11 October 2014

Abstract:

Diagnosis of pulmonary aspergilloma is based on chest radiographic
images (fungus ball) and specific antibody detection by serologic pro-
cedures. Concerning Aspergillus serology the reference method
remains detection by immunoprecipitation although this method
lacks standardization.
Objective To compare the commercially available IMB kit “œ Asper-
gillus WB IgG”(LBDBio Diagnostics, Lyon, France) with the immuno-
precipitation method used in our laboratory. A panel of sera from
patients with proven aspergilloma (lung biopsy showing mycelium
and culture of Aspergillus fumigatus) was tested using the two meth-
ods in parallel.
Methods We used a total of patient 32 sera. Nine were collected at
our hospital and the 23 sera were provided by 4 other hospitals. IMB
was used in accordance with manufacturer’s instruction. A serum
was considered as positive if a minimum of 2 bands were detected
among the 4 specific Aspergillus antigen bands (16, 18-20, 22,
30 kD). IPD used commercial somatic and metabolic antigens (Bio-
Rad, Marne-La-Coquette, France) and a commercial Aspergillus posi-
tive control serum (bioRad,Marne-La-Coquette,France). A serum was
considered as positive if one somatic or metabolic antigen was
precipitated.
Results Of these 32 sera 19 were positive with IPD (detection from
one to nine antigens). The sensitivity was 59%. Thirty-three sera
were positive with IMB (detection from 2 to 4 bands). The two bands
the most often observed were P16 and P18-20. The sensitivity was
97%. IMB showed significantly higher sensitivity than IPD. Specificity
of IMB previously performed on blood donors (n = 213) was evalu-
ated to 96%. IMB offers important advantages over IPD: small
amounts of serum are required (15 ll versus 60 ll) and in contrast
to IPD the criteria for interpreting positivity is simpler. The antigen-
positive bands are much easier to detect than precipitated antigens.
Conclusion IMB appears a better method to diagnose pulmonary
aspergilloma.

Abstract Number: p058

Conference Year: 2013

Link to conference website: NULL

New link: NULL

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