Development of Resistance to Agricultural Azole Antifungal Compounds – Emergence of Multidrug Cross-Resistance Among Human Fungal Pathogens Candida, Cryptococcus and Aspergillus

Ref ID: 18684

Author:

I. Faria-Ramos, MS – Researcher 1, S. Farinha, MS – Researcher 1, P. Tavares, MS – Researcher 1, J. Maia, MS – Researcher 1, E. Ricardo, MS – Researcher 1,2, I. M. Miranda, PhD – Researcher 1,2, C. Pina-Vaz, MD, PhD – Researcher 1,2,3, A. G. Rodrigue

Author address:

1Faculty of Med., Univ. of Porto, Oporto, Portugal, 2Cardiovascular Res. & Dev. Unit, Faculty of Med., Univ. of Porto, Porto, Portugal, 3Hosp. S. João, Porto, Portugal.

Full conference title:

52nd Annual ICAAC

Date: 9 September 2014

Abstract:

Background: Clinically relevant fungal infections became progressively more prevalent during the last two decades and resistance to antifungals arises as a major clinical problem. Antifungals similar to those used in human therapy are widely used in agriculture and resistance to such antifungals is also increasing. The acquisition of azole resistance in nature may thus result in a significant, yet undetermined, impact regarding human health. The main goal of this work was the assessment of development of cross-resistance between agricultural and clinical azoles. Methods: Candida albicans, C. parapsilosis, C. glabrata, Cryptococcus neoformans and Aspergillus fumigatus strains with a susceptible phenotype to clinical azoles were daily incubated at 35°C, 150 rpm with sub-inhibitory concentrations of prochloraz (PCZ). PCZ minimal inhibitory concentrations (MIC) was evaluated, according to the CLSI M27-A3 and M38-A protocols every five days. Whenever a significant increase of PCZ MIC values was achieved, the MIC of antifungals used in the clinical setting (fluconazole (FLU), voriconazole (VOR), posaconazole (POS)) was determined. Results: The development of resistance to PCZ was evident, with cross-resistance to clinical azoles (Table 1). Conclusions: The exposure of clinical relevant fungi to agricultural azoles is associated to the emergence of cross-resistance to clinical azoles, which are crucial in hospital settings. *Intrinsically resistant to FLU

Abstract Number: M-320

Conference Poster: y

Conference Year: 2012

Link to conference website: NULL

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