Development of a homologous protein carrier system for heterologous protein production in Myceliophthora thermophila.

Ref ID: 18536

Author:

Hans Visser, Sanaz Mokhtari, Jan Wery

Author address:

Dyadic Nederland BV

Full conference title:

11 th European Conference on Fungal Genetics

Abstract:

Filamentous fungi have proven to produce and secrete large quantities of extracellular enzymes. Species such as
Aspergillus niger, Aspergillus oryzae, Trichoderma reesei and Myceliophthora thermophila  C1 are used in industry
as work horses for enzyme production. High yields of homologous enzymes   can be readily obtained. On the
contrary, heterologous proteins are often produced at low levels. One of the main reasons for this is the presence
of host proteases that partially or fully degrade the heterologous protein.  In addition  at the level of transcription,
translation and transport and processing through the secretion pathway problems may be encountered. In order
to increase the chances of success, protein carriers have been used for improved heterologous protein production
in filamentous fungi
1
. It is believed that the carrier protein will “œdrag/guide” the heterologous protein through the
(initial stages)  of the secretion pathway protecting it from mis8208;folding and proteolysis. Previously, we have used
this technology successfully in the expression of human antibodies in C1
2
.  The carrier protein used in that study
was the catalytic domain of Aspergillus niger glucoamylase A, which by itselfis a heterologous protein to C1.  In the
present study we investigated whether the homologous C1 glucoamylase yields higher levels of a heterologous
xylanase sensitive to  proteolysis.  Â 
1
Gouka et al. (1997) Efficient production of secreted proteins by Aspergillus:
progress, limitations and prospects. Appl. Microbiol. Biotechnol. 47: 18208;11.
2
Visser et al. (2011) Development of a
mature fungal technology and production platform for industrial enzymes based on a Myceliophthora thermophila
isolate, previously known as Chrysosporium lucknowense C1. Ind. Biotechnol. 7(3): 2148208;223.

Abstract Number: PR8.65

Conference Year: 2012

Link to conference website: http://www.ecfg.info/images/Abstract_Book_Electronic.pdf

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