Aspergillus fumigatus is a saprotrophic filamentous fungus capable of causing a number of infectious respiratory diseases in susceptible individuals. The initial stages of infection by A.
The human bronchial epithelial cell line (1HAE) was grown to confluence in 24-well plates with Dulbecco's Modified Eagle's Medium (DMEM) plus 10% of fetal bovine serum (FBS). A.
Cells-only and cells treated with spores could be differentiated as distinct populations based on GFP fluorescence. Potential internalization events of single spores were observed in single 1HAE cells, using appropriate gating in FACS, approximately 50% of the time. Attachment of spores to the cell surface was also observed.
We have successfully developed a single host cell-single spore model. Single spore internalization events were enriched and imaged by microscopy,
Full conference title:
- ATS 2018