Detection of (1,3)-beta-d-glucan In Bronchoalveolar Lavage Fluid For the Diagnosis of Invasive Aspergillosis

Ref ID: 18734

Author:

A. Atalay, M.D – Assistant Professor, G. Metan, MD (Doctor of Medicine) – Associate Professor, A. N. Koc, M.D – Professor, L. G. Kaynar, M.D – Assistant Professor, H. Buyukoglan, M.D – Assistant Professor, I. Bozkurt, M.D – Infectious Diseases Specia

Author address:

Erciyes Univ., Kayseri, Turkey.

Full conference title:

52nd Annual ICAAC

Date: 9 September 2014

Abstract:

Background: The aim of this study is to assess the utility of (1,3)-beta-D-glucan (BDG) assay in bronchoalveolar lavage (BAL) fluid for the diagnosis of invasive aspergillosis (IA). Patients and methods: Forty-five patients at high risk for IA and underwent bronchoscopy were included in the study. Twenty-three of the patients had hematological malignancies. 15 out of 45 patients had probabe IA based on European Organization for Research and Treatment of Cancer and the Mycoses Study Group criteria (independent of BDG results). Serum samples and BAL fluid were tested for BDG (Fungitell; Associates of Cape Cod Inc, Falmouth, MA,USA) according to the manufacturers’ recommendations. Results: The sensitivity of BDG in BAL fluid was 93.3% (95% Confidence interval (CI), 66-99.6), specificity was 53.3% (95% CI,34.6-71.2), positive predictive value (PPV) was 50%, and negative predictive value (NPV) was 94.1% at a cutoff value of 80 pg/mL, respectively. An Receiver operating characteristics (ROC) curve was constructed to define the optimum BAL BDG cutoff value for the diagnosis of IA (Figure 1). The area under the ROC curve was 0.81 (95% CI, 0.66-0.91; p<0.001). At a cutoff value of 150 pg/mL, the sensitivity was 80% (95% CI, 51.9- 95.4), specificity was 76.7% (95% CI, 57.7- 90.0), PPV was 63.2%, and NPV was 88.5%. Conclusion: Detection of BDG in BAL fluid seems to be a promising tool to rule out IA in high risk patients based on the high NPV.

Abstract Number: M-1701

Conference Poster: y

Conference Year: 2012

Link to conference website: NULL

New link: NULL

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