DenA is a deneddylating protein involved in A. nidulans development

Ref ID: 18362


t Martin Christmann, Rebekka Harting, Özgür Bayram, Gerhard H. Braus,

Author address:

Institute for Microbiology and Genetics, Georg August University Göttingen, D-37077 Göttingen, Germany (Germany),

Full conference title:

Asperfest 8


Deneddylation is the removal of the ubiquitin (Ub)-like protein Nedd8 from cullins. Cullins are subunits of cullin-RING Ub ligases (CRL) which are
controlled in their activity and assembly/reassembly by neddylation and deneddylation, respectively. The most important eukaryotic deneddylases are
the COP9 signalosome (CSN) and the deneddylating enzyme 1 (DEN1). Mammalian Den1 has two functions: an isopeptidase activity removing Nedd8
from cullins and other proteins and an additional linear peptidase activity processing Nedd8 from a precursor protein. Filamentous fungi possess an eight
subunit COP9 signalosome (CSN) which is reminiscent to the corresponding plant and vertebrate complex (Busch et al. 2007, Braus et al., 2010).
Aspergillus nidulans requires CSN function to trigger development, the appropriate response ofthe fungus towards light, and for a coordinated secondary
metabolism (Nahlik et al., 2010). We show here the characzterization of the fungal Den1 ortholog DenA. The denA gene encodes a cysteine protease
deneddylating enzyme. DenA is required for light control and the asexual fungal development whereas CSN is required for the sexual cycle. Processed
Nedd8 is unable to rescue conidia formation suggesting that the lack of the DenA deneddylase isopeptidase activity is responsible for the defect. Yeast
two hybrid studies suggest a physical interaction between DenA and CSN which has to be further evaluated. Busch S, Schwier EU, Nahlik K, Bayram
í–, Draht OW, Helmstaedt K, Krappmann S, Valerius O, Lipscomb WN, Braus GH (2007), PNAS, USA. 104, 8125-8130. Braus GH, Irniger S, Bayram
í– (2010), Curr. Opin. Microbiol. 13, 1-5. Nahlik K, Dumkow M, Bayram í–, Helmstaedt K, Busch S, Valerius O, Gerke J, Hoppert M, Schwier E, Opitz
L, Westermann M, Grond S, Feussner K, Goebel C, Kaever A, Meinecke P, Feussner I, Braus GH (2010), Mol. Microb. 78, 962-979.

Abstract Number: 40)

Conference Year: 2011

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