D8208;xylose Concentration8208;dependent Hydrolase Expression Profiles and the According Role of CreA and XlnR in A. niger

Ref ID: 18515

Author:

Astrid R. Mach-Aigner,
Jimmy Omony,
Birgit Jovanovic,
Anton J.B. van Boxtel
Leo H. de Graaff

Author address:

Research Division Biotechnology and Microbiology, Institute of Chemical Engineering, Vienna University of
Technology, Gumpendorfer Str. 1a, A-1060 Vienna, Austria  
Systems and Control group, Wageningen University,
P.O. Box 17, 6700 AA Wagen

Full conference title:

11 th European Conference on Fungal Genetics

Abstract:

Aspergillus niger is an industrially important organism for the production of industrial enzymes like hemicellulases
and pectinases. The xylan8208;backbone monomer D8208;xylose is known as an inducing substance for the coordinate
expression of a high number of polysaccharide8208;degrading enzymes. In this study a total number of 22 genes, which
encode enzymes that function as xylan backbone8208;degrading enzymes, accessory enzymes, cellulose8208;degrading
enzymes, or enzymes involved in the pentose catabolic pathway in A. niger have been investigated concerning
their response to low (1 mM) and high (50 mM) D8208;xylose concentrations. Notably, genes encoding enzymes that
have similar function (e.g. xylan backbone8208;degradation) respond in a similar way to different amounts of D8208;xylose.
Although low D8208;xylose concentrations provoke  8208;  in particular for hemicellulase8208;encoding genes  8208;  highest
transcription response, transcript formation in presence of high amounts of D8208;xylose was also observed. It even
turned out that a high D8208;xylose concentration is favourable for certain groups of genes. Furthermore, the
repressing influence of CreA on the transcription of a selection of these genes was observed on D8208;xylose,
regardless whether low or high amount of D8208;xylose is used. Interestingly, the decrease in transcription of certain
genes on high D8208;xylose concentrations is not reflected by transcription of their activator XlnR. Regardless of the D8208;
xylose concentration applied and whether CreA was functional or not, xlnR was constitutively expressed at a low
level.

Abstract Number: PR8.12

Conference Year: 2012

Link to conference website: http://www.ecfg.info/images/Abstract_Book_Electronic.pdf

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