D8208;galactose uptake of Aspergillus niger

Ref ID: 18539

Author:

Erzsébet Fekete,
Christian P. Kubicek,
Ronald P. de Vries,
Bernhard Seiboth,
Erzsébet Sándor,
Patricia A.
vanKuyk,
Levente Karaffa

Author address:

Department of Biochemical Engineering, Faculty of Science and Technology, University of Debrecen, Hungary

Research Area Biotechnology and Microbiology, Institute of Chemical Engineering, TU Wien, Austria

Microbiology,
Utrecht University, Pad

Full conference title:

11 th European Conference on Fungal Genetics

Abstract:

The majority of Black Aspergilli (Aspergillus Section Nigri), including Aspergillus niger, as well as many other
Ascomycetes fail to germinate on D8208;galactose as a sole carbon source. Here, we provide evidence that the ability
of A. niger to transport D8208;galactose is growth stage dependent, being absent in the conidiospores but partially
present in the mycelia. Despite earlier claims, we could identify galactokinase activity in growing cells and all genes
of the Leloir8208;pathway (responsible for channeling D8208;galactose into the EMP8208;pathway) are well induced on D8208;
galactose (and also on lactose, D8208;xylose and L8208;arabinose) in the mycelial stage. Expression of all Leloir pathway
genes was also detectable in conidiospores, though galE (encoding a galactokinase) and galD (encoding a UTP8208;
galactose8208;18208;phosphate uridylyl transferase) were expressed very poorly. These results suggest that the D8208;
galactose8208;negative phenotype of A. niger conidiospores is due to the lack of inducer uptake.  

Abstract Number: PR8.73

Conference Year: 2012

Link to conference website: http://www.ecfg.info/images/Abstract_Book_Electronic.pdf

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