CoPacC, pH8208;responsive transcriptional factor, is involved in the entry mode selection of Colletotrichum orbiculare at wounded sites of Arabidopsis leaves.

Ref ID: 18508

Author:

Kae Yoshino, Tetsuro Okuno, Yoshitaka Takano

Author address:

Kyoto University 

Full conference title:

11 th European Conference on Fungal Genetics

Abstract:

Colletotrichum orbiculare (Co) is the causal agent of cucumber anthracnose disease. Normally, Co forms melanized
appressoria which enable the fungus to invade the plant. However, we have recently found that Co exhibits hyphal
tip8208;based entry (HTE), uncoupled with melanized appressoria, at wounded sites of Arabidopsis leaves. To
investigate what kinds of signals at wounded sites induce HTE, we looked for the in vitro conditions that can trigger
HTE. As a result, we found that appressorium development of Co on hydrophobic surface was suppressed when
ambient pH was shifted to alkaline condition. PacC is known to pH8208;responsive transcriptional factor in several
filamentous fungi. To assess the potential involvement of PacC in switching to HTE, we identified the PacC
homolog of Co and generated CopacC null mutants. Remarkably, at wounded sites of Arabidopsis leaves, the ratio
of melanized appressorium formation of the CopacC mutants significantly increased in comparison with that of the
wild type. Furthermore, the transudate collected from wounded sites of Arabidopsis leaves induces HTE8208;like
morphogenesis of Co in vitro in the CoPacC8208;dependent manner, and its activity severely decreased when the
transudate pH was altered. It was reported that PalC is the activator of PacC and localizes to cortical structures
when pH signalling is active in Aspergillus nidulans. To assess the activation of pH signalling during HTE8208;like
morphogenesis and appressorium development of Co, we have generated a PalC GFP8208;tagged mutant. Our results
suggest that the PacC8208;mediated pH sensing and regulation is involved in the entry mode selection of Co.  

Abstract Number: PR7.35

Conference Year: 2012

Link to conference website: http://www.ecfg.info/images/Abstract_Book_Electronic.pdf

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