Contribution to the knowledge of biodiversity of Aspergillus species in Iran

Ref ID: 19308

Author:

Bita Asgari, Rasoul Zare

Author address:

Department of Botany, Iranian Research Institute of Plant Protection, P.O. Box 1454, Tehran 19395,
Iran

Full conference title:

Asian Mycological Congress 2013 and the 13th International Marine and Freshwater Mycology Symposium

Date: 19 August 2014

Abstract:

The genus Aspergillus accommodates important species in industry, medicine and human
health. Taxonomic problems of the genus lie in the limited choice of morphological, developmental, and
cultural characters, with a wide range of particularly phenotypic variation. The current study was aimed to
evaluate the biodiversity of Aspergillus species on various agricultural crops in Iran using a polyphasic
approach. Some 540 strains isolated from cereals (wheat, barley and maize) and 46 isolates recovered
from banana, cucumber, kiwi fruit, melon, peanut and pistachio were examined. To select representative
strains for sequencing, the amplified parts of the β -tubulin gene of all strains were subjected to
PCR-RFLPs. Through this method, some 100 isolates were selected and sequenced in ITS nrDNA,
partial β -tubulin and partial calmodulin regions. All these strains were also studied morphologically after
they were grown on standard media. Based on morphological and molecular data, 42 species of
Aspergillus could be identified, falling into sections Aspergillus, Candidi, Circumdati, Flavi, Flavipedes,
Nidulantes, Nigri, Usti, Versicolores and Wentii. Forty-nine percent of isolates recovered in this study are
assigned to section Aspergillus. Among the identified species, 18 are new records for the Iranian
mycobiota. Besides, 10 species are newly discovered and will be officially described and published in
due course. A new teleomorph is also described for the purely anamorphic species of section Aspergillus,
A. proliferans. Partial β -tubulin and partial calmodulin regions are proposed as the best phylogenetic
marker and DNA barcode for species recognition of Aspergillus, respectively.

Abstract Number: s1-2-17

Conference Year: 2013

Link to conference website: NULL

New link: NULL

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