Conditional Gene Expression in Aspergillus niger - Perspectives and Limitations of Inducible Expression Systems

Simon Boecker, Franziska Wanka, Roderich Süßmuth, Mark Arentshorst, Arthur Ram, Vera Meyer

Abstract: 

Synthetic biology tools allowing precise regulation of gene expression are powerful tools to study gene functions in vivo and to streamline metabolic pathways. Inducible, tuneable and metabolism-independent gene switches are of special interest as they can be easily controlled and shut on or off at any stage during the life cycle of the organism of interest. Recently, we established a tetracyclin-dependent expression system for the industrial platform organism Aspergillus niger that can either be used to switch the expression of a certain gene on (the Tet-On system) or off (the Tet-off system) upon addition of the inducer tetracycline or its derivative doxycyclin.[1,2] However, if more genes have to be controlled independently, this system reaches its limits. We therefore aimed to design additional inducible expression systems functioning in A. niger and other filamentous fungi. We tested the performance and efficacy of two conditional expression systems responding to the antibiotics inducer erythromycin or to the hormone analog diethylstilbestrol. We used luciferase as a reporter gene and applied a MTP-based assay to evaluate and compare the systems with the established Tet-On and Tet-off systems [1,2]. Corresponding results will be shown.

[1] V. Meyer et al. (2011) Appl. Environ. Microbiol., 77, 2975–2983.[2] F. Wanka et al. (2015) Fungal Genet. Biol., in press.

2016

abstract No: 

3

Full conference title: 

The Thirteenth International Aspergillus Meeting, EUROSITES La Chapelle, PARIS, FRANCE
    • Asperfest 13 (2016)