The filamentous fungus Acremonium chrysogenum is the primordial producer of the β-lactam antibiotic cephalosporin C. This β-lactam antibiotic is of great biotechnological and medical relevance due to its antibacterial activity against gram-positive and -negative bacteria. Continuous and directed improvement of industrial strains is required to optimize cephalosporin C production efficiently. A typical morphological feature of A. chrysogenum is the fragmentation of vegetative mycelium into arthrospores. These are uni- or binuclear cells, which develop during a prolonged cultivation under limited nutrient supply. Due to the known correlation of cephalosporin C production and arthrospore formation, we are interested in the identification of specific regulatory factors affecting both, cephalosporin C biosynthesis and morphological development. Current analyses focus on septation because constriction of arthrospores occurs from septa. Here, we present functional characterizations of components of the bud site selection system and the septation initiation network. We performed microscopic analysis of arthrospore formation in different time intervals, growth tests under various stress conditions and localization studies to verify the direct regulation of arthrospore formation by this regulatory network. To further investigate this developmental process, we established the visualization of filamentous actin with the live cell marker Lifeact.
Full conference title:
- Asperfest 13 (2016)