Comparison of aspergillus precipitin with quantitative aspergillus IgG assay

Ref ID: 19499

Author:

Y Fujita1*, S Fujiuchi1, H Suzuki1, T Aritomi1, H Kuroda1, M Takahashi1, A Takeda1, Y Yamazaki1,
T Tsuji1, T Fujikane1

Author address:

1Department of Respiratory Medicine, NHO Asahikawa Medical Center, Asahikawa, Japan

Full conference title:

6th Advances Against Aspergillosis 2014

Abstract:

Purpose:
Detection of IgG antibody to Aspergillus fumigatus is important for diagnosing chronic pulmonary
aspergillosis. Immunodiffusion assay has been widely used for detecting Aspergillus precipitin,
however, it is seems to be time consuming and lack of sensitivity. Recently, quantitative measurement
of IgG to Aspergillus by using fluorescent immunoenzyme assay (ImmunoCapTM) is commercially
available. In this study, we examined performance of ImmunoCapTM for detecting Aspergillus
precipitin.
Methods:
From January 2007 to August 2013, we determined serum Aspergillus precipitin for 353 consecutive
patients who suspected chronic pulmonary aspergillosis. We also measured specific IgG to Aspergillus
for these collected sera by ImmunoCapTM (Phadia,Uppsala). The patients with history of antifungal
treatment were excluded.
Results:
There were 249 male and 104 female. Median age was 72.6 years. One hundred and twenty-six
patients (35.7%) were positive for Aspergillus precipitin. Aspergillus IgG level was significantly
higher in the precipitin positive group (148.2mgA/L) than in the precipitin negative group
(39.8 mgA/L) (p <0.01). ROC analysis revealed that cut off value of ImmunoCapTM for detecting Aspergillus precipitin was 50mgA/L. (AUC 0.83, sensitivity0.78, specificity0.76). Conclusion: The sensitivity and specificity of ImmunoCapTM for detecting Aspergillus precipitin was acceptable. Detecting circulating Aspergillus antibody by ImmunoCapTM in the early stage of disease may result in relatively high false positive rate (0.36). Thus, ImmunoCapTM may alternate with conventional precipitin test for diagnosing chronic pulmonary aspergillosis. In addition to it, the quantitation of IgG value might be useful for monitoring marker of antifungal treatment response.

Abstract Number: 27

Conference Year: 2014

Link to conference website: http://www.AAA2014.org

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