Characterization of Fumonisin B2 biosynthetic gene cluster in A. niger and A. awamori

Ref ID: 18261

Author:

Antonia Susca
, Robert H. Proctor
, Giuseppina Mule
, GAETANO Stea
, Miriam Haidukowsky
, Antonio Logrieco
, and Antonio
Moretti

Author address:

Institute of Sciences of Food Production, CNR, via Amendola 122/0, 70126 Bari, Italy,
USDA ARS National Center for
Agricultural Utilization Research, 1815 North University Street, Peoria, Illinois 61604-3902 antonella.susca@ispa.cnr.it

Full conference title:

Asperfest 9

Abstract:

A set of 43 Aspergillus niger and A. awamori strains isolated from grapes cultivated in Mediterranean area was tested to evaluate
eventual genetic differences between fumonisin B2 (FB2) producing and non-producing strains. The ability to produce FB2 was
correlated to fum genes occurrence, evaluated by PCR assyas, using primer sets designed to amplify fragments of fum1, fum3, fum6,
fum7, fum8, fum10, fum13, fum14, fum15, and some relative intergenic regions. The A. niger and A. awamori FB2 producing strains
arose amplicon for all tested fum genes, while the FB2 non-producing strains arose amplicon only for few of tested fum genes in A.
awamori, and for all of them in A. niger. Maximum parsimony analysis based on the calmodulin gene sequences indicated that the
presence/absence of fum genes in the isolates is not correlated with phylogenetic relationship among strains. This is the first report
correlating the presence of multiple fumonisin biosynthetic genes with fumonisin production in A. niger. The results suggest that the
absence of FB2 production in A. awamori can result from the absence of at least one gene of the cluster, while in A. niger should
involve other regulator gene/s probably out of the cluster, or concern variations in a regulatory sequence essential for cluster
expression.

Abstract Number: 6)

Conference Year: 2012

Link to conference website: NULL

New link: NULL

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