Characterization of farA and other zinc finger transcription factor genes for fatty acid metabolism in Aspergillus oryzae

Ref ID: 18480

Author:

Sharon Marie Garrido,
Noriyuki Kitamoto,
Akira Watanabe,
Takahiro Shintani,
Katsuya Gomi

Author address:

Graduate School of Agriculture, Tohoku University, Sendai City, Japan  
Food Research Institute, Nagoya, Japan

Full conference title:

11 th European Conference on Fungal Genetics

Abstract:

The zinc finger transcription factor FarA in Aspergillus nidulans up8208;regulates genes required for growth on fatty
acids (Hynes et al, 2006). Ctf1 and Por1, orthologs of FarA of A. nidulans are required for growth on fatty acids in
Candida albicans and also for the essential transcriptional activation of genes involved in beta8208;oxidation and
peroxisomal biogenesis in Yarrowia lipolytica, respectivel (Ramirez and Lorenz, 2009; Poopanitpan et al, 2010).
FarA transcriptional factor is also found in the Aspergillus oryzae which has 83% homology of all the amino acid
sequences and 97.5% homology of Zn2Cys6 motifs with the A. nidulans. In this study, farA disruptant in A. oryzae
was characterized and expression levels of genes for fatty acid metabolism were also determined.
Interestingly,A.oryzae farA disruptants showed indistinguishable growth in fatty acid sources compared to thewild8208;
type, inconsistent with the growth phenotype of the A. nidulans counterpart. In contrast, expressions of some
genes for fatty acid metabolism were significantly reduced in the farA disruptants. These contradicting results
suggested that FarA may act not only the primary transcriptional activator for fatty acid utilization in A. oryzae and
that another transcriptional factor(s) may regulate other fatty acid metabolic genes which can be accounted to the
differences on the number of genes between these two Aspergilli. We then proceeded to screen other zinc finger
transcription factor gene disruptants from the disruptant library of A.oryzae for fatty acid metabolism.
Characterization and implication of these disruptants in response to different fatty acid substrates and fatty acid
gene expressions are currently underway.

Abstract Number: PR3.21

Conference Year: 2012

Link to conference website: http://www.ecfg.info/images/Abstract_Book_Electronic.pdf

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